The University of Wisconsin-Stevens 
Point has established a new Genetic Analysis Service (GAS) available
for UWSP faculty and students. The Department of Biology has
a new Applied Biosystems 310 Capillary Genetic Analyzer that is
housed in the newly renovated Genetics Lab in room B212 of the
Science Building, to be used for both teaching and research purposes.
The system can determine the DNA sequence of any part of any
genome, from viruses to humans. In addition, it can generate
DNA fingerprints from any organism and without any prior knowledge
of its DNA. These services can be used in a wide variety of disciplines,
such as gene identification, mutation detection, genetic relatedness,
genetic disorders, disease detection, species identification,
and evolution.
Cycle-Sequencing and Electrophoresis Service:
If
you have a polymerase chain reaction (PCR) product that you want to get
sequenced, purify the DNA PCR reaction using the Qiagen PCR
purification kit. Then quantify your DNA using a fluorometer. Samples
can then be sequenced for you for $20 per sample. This charge is used to
defray the costs of cycle-sequencing reagents, sample purification and
capillary electrophoresis.
Click on the following protocol links to download your own copy of the directions.
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